Number One (Star Trek)
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Species: Human
Gender: Female
Home planet: Unknown
Affiliation: Starfleet
Position: USS Enterprise executive officer
Rank: Lieutenant
Portrayed by: Majel Barrett (as M. Leigh Hudec)

Number One, in "The Cage", the original pilot episode of the science fiction television series Star Trek, was the un-named intellectual, problem-solving second-in-command serving under Captain Christopher Pike. Number One is the naval term for the First Officer on board a ship, second-in-command to the captain. During "The Cage," Number One proves to her alien captors that humans would rather die than be slaves.

Played by Majel Barrett, who went on to play Nurse Christine Chapel in the original Star Trek and Lwaxana Troi in Star Trek: The Next Generation, as well as the computer's voice), the character appears only in the unaired pilot and in the footage used in "The Menagerie". In 2019, Number One appears in the second season of the prequel series Star Trek: Discovery, played by Rebecca Romijn.